THE EFFECT OF PHYLLANTHUS ACIDUS [ L.] SKEELS LEAF EXTRACT ON IGE LEVEL IN MICE MODEL OF ALLERGY

AA Subijanto¹ and Diding HP²
1 Sebelas Maret University School of Medicine, Solo
2 Immunology Division of Biomedic Laboratory, Sebelas Maret University School of Medicine, Solo

ABSTRACT

It was reported in previous studies that pharmacological activities were identified in various compounds extracted from Phyllanthus Acidus (PA) such as anti-inflammatory, anti-lipoxigenase, antiallergic and cyclooxigenase inhibitor. It is also acknowledged that Ig E serum level is normally low and usually increase in allergic conditions. This studywas aim to determine the effect of PA leaf extract on Ig E level in mice model of allergic ashtma. Male Balb/C mice were sensitized and challenged intraperitoneally (i.p) with ovalbumin (OVA). Mice were immunized i.p. on days 4 and 20 with 2.5 mg of OVA adsorbed to 7.75 ml of Aluminum hydroxide gel. OVA challenges (10 mg in l0 ml of PBS) were administered aerosolly on days 26, 29, 32, 39 and 46, with mice being sacrificed on day 47. Ig E level in serum was determined using ELISA kits (Alpha Diagnostic, USA) according to the manufacturer's instructions. A one-way analysis of variance (ANOVA) with LSD post hoc analysis was used to determine significant differences. Results are expressed as mean ± SEM, and values of p < 0.05 were considered statistically significant. The result showed that Ig E level in the negative control group was 50.13±33.40 ng/ml, PA leaf extract was 51.17±26.15 ng/ml, and positive control group was 48.97±33.50 ng/ml while OVA group was 152.89±58.00 ng/ml (p >0.05). It was concluded that PA leaf Extract could minimized Ig E level, not significantly difference with 3rd generation of antihistamine.

Keywords : Phyllanthus Acidus; leaf extract; Ig E; allergic condition.
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