The Effect of Lactobacillus reuteri on Survival, Intestinal Inflammation and Ig A level in Mice Model of Sepsis

Diding H.P.1

1Immunology Division, Biomedical Laboratory School of Medicine, Sebelas Maret University, Surakarta

Objective: This study was aimed to evaluate the effect of L. reuteri on survival, intestinal inflammation and Ig A serum level in mice model of sepsis.
Methods: Male Balb/C mice were inoculation with cecal inoculum for mice model of sepsis. Mice model of sepsis grouping to sepsis, sepsis with L. reuteri and control. Detection of intestinal inflammation with HE staining on 8th day. Survival perceived until 8th day. Ig A level measurement with Elisa. Two-Tailed Fisher exact test for the analysis of survival, Kruskal- Wallis test for the intestinal inflammation, One-way Anova for Ig A level and p < 0.05 were used to determine significant differences.
Results: L. reuteri showed no significantly mortality (10% vs 40%, p =0.065) compared to sepsis. Intestinal inflammation in sepsis group were grade 2nd (11.1%), grade 3rd (55.6%) and grade 4th (33.3%), while L. reuteri group showed grade 0 (50.0%), grade 1st (33.3%) and grade 2nd (16.7%) respectively, with p =0.000. L. reuteri showed significantly of Ig A level (126.05±34.26 ng/mL vs 6.20±5.80 ng/mL, p =0.000) compared to sepsis.
Conclusion: This study concludes that L. reuteri can decrease intestinal inflammation, on the other hand increase survival and Ig A level in mice model of sepsis.


Keywords : L. reuteri; sepsis; Ig A; inflammation
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